BioNTech Investor Day Presentation Deck

Synthetic (endo)lysins - A potentially ideal class of precision antibacterials Used by phages to degrade bacterial cell wall Gram- positive Gram- negative Bacteriophage Endolysin Bacterial killing Modular domain architecture Outer-membrane penetrating peptide High diversity in architectures and combinations Enzymatically active domain AF Cell-wall binding domain Enzymatically active domain Highly potent No resistance Biofilm active Laser focus Safe ● ● ● Highly bactericidal Minimum inhibitory concentration (MIC) often <1 µg/ml Active on antibiotics-resistant bacteria Resistance formation hardly possible Lyse cell-wall irrespective of metabolic state Penetrate biofilm matrix Do not harm beneficial bacteria Suitable where microbiome has to be preserved Mammals have no peptidoglycan Very safe, no off-target effects (Endo)lysins could be developed against virtually any type of bacteria Antibacterials 1 Fischetti VA. Int J Med Microbiol 2010; 300:357-362; 2 Vázquez R, et al. J Virol 2021; 95:e0032121; 3 Fowler VG, et al. J Clin Invest 2020; 130:3750-3760. ~D BIONTECH 85

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