Cytek Investor Day Presentation Deck
Cytek Biosciences Aurora CS cell sorter
Human PBMCs, 14 color panel (T cell subsets including naïve/memory and Treg)
Sorting for CD4+ and CD8+ populations
UNSORTED
CD8+
CD45RA+
SORTED
Percentage
target
population
2.9%
CD8+
CD45RO+
SORTED
Percentage
target
population
3.2%
side scatter
side scatter
side scatter
OM-
4CM-
CM-
1.0M
2.0M
Lymphocytos
68 8
1.0M
forward scatter
2.0M
1.0M
Lymphocytes
792
3.0M
2.0M
3.0M
forward scatter
4.0M
3.0M
4.0M
4.0M
forward scatter
SSC-A
4CM-
3 CM-
2 CM-
side scatter H
10M-
4.0M-
3.0M-
1.0M-
-104
CD45+ Dalle
99 2
side scatter A
CD45+ cels
99.8
10
10
side scatter A
CD45+ colo
100
10"
10⁰
m
side scatter A
Como-Alexa Fluor 488
side scatter A
side scatter A
T
D
59.5%
CD3+CD2-
59.5
hCD45 eF450
10
10² ID
CD3+ CD2-
97.4
97.4%
104
hCD45 eF450
10°
CD3+CD2-
99.4
99.4%
10°
hCD45 eF450
Comp-Odot 655-A CD8
hCD2 AF488
hCD2 AF488
CD8+
48.3
€48.3%
CD4+
45.0
45.0%
10
CD8+
99.2
hCD3 BUV395
CD4+
0.82
10⁰
99.2%
hCD3 BUV395
CD8+
99.7 99.7%
CD4+
0.24
10
10⁰
hCD3 BUV395
108
hCD8 QD650
hCD8 QD650
CD8+ CD45RA+
35.3
CD8+ CD45RO+
39.2%
www.
-10°
CDB+ CD45RA+
92.6
10%
hCD4 R-PC
CD8+ CD45RO+
6.41
CDB+ CD45RA+
0.31
10°
CD8+ CD45RO+
0
:35.3%
98.6%
10
hCD4 R-PC
96.6%
10°
10
hCD4 R-PC
unlabeled
Alexa Fluor 488 hCD2
PE hCD56
R-PC hCD4
PECY7 hCD45RA
APC hCD19
APCCy7 hCD45RO
eF450 hCD45
BV605 hCD20
BV650 hCD25
QD655 CD8
BV711 hCD127
QD800 hCD14
BUV395 hCD3
BUV661 hCCR7
Sort purity
96.6%
=
Actual yield
(actual over
calculated)
70.3%
Sort purity=
98.6%
While still being fully optimized, we now have spectral cell sorting capability, a
necessity for our group.
=
Actual yield
(actual over
calculated) =
71.3%View entire presentation