Immix Biopharma Investor Presentation Deck slide image

Immix Biopharma Investor Presentation Deck

N-GENIUS Platform Process 1. Problem Identification: CAR-T Toxicity Main Block To Widespread Use: Neurotoxicity 10. NXC-201 4-188 CD3 2. Research Group Formation: Reduce CAR-T Toxicity While Improving Efficacy Day-1 Day +3 Day +7 9. Evaluation CAR-T activity in vivo (tumor growth, cytokine release, safety evaluation) Day +11 Day +14 Day +20 Day +28 Day +56 Day +76 NT Mouse 1 Mouse 2 NT Mouse 3 Mouse 4 Source:: Harush O, et al. Haematologica. 2022; Tumor 1.04g 171g 1.50 HBBB 5x10 za HBBB 10x10 H8BB 15x105 3. Platform Foundation: N- GENIUS Platform with EXPAND Technology aims to achieve research group goal NEXCELLA NEXT GENERATION CELL THERAPIES 8. Co-culture of CAR-T cells and target cells with target antigens and in vitro analysis of cytokine release www. 4. Candidate Creation: Entire CAR- T construct optimized with N- GENIUS Platform and EXPAND Technology In Selected Indications 7. CAR-T Transgene expression was assessed by flow cytometry % of Max 0 10³ CAR NGFR, APC 67% 62% 40% 41% T™ 10* H8BB 5. Final Candidate Selection: 4 promising candidates selected among a larger number of options H828 ICBB IC28 ●●● S Anti BCMA scFv Anti BCMA scFv Anti BCMA scFv Anti BCMA scFv Blood collection (cukapheress) IMMİX BIOPHARMA CD8a CD8a hinge TM CD8a hinge CD8a TM IgG4 CD28 hinge TM IgG4 CD28 hinge TM Continuous flow centrifugation 41BB CD28 Retum unneeded blood components 41BB CD28 Manarucles cels CD3 6. Human Peripheral blood mononuclear cells (PBMC) were transduced with the CAR candidates CD3 CD3 CD3 Plack por plasma Endalend cells and granulocytes Isalate PBMC fraction 62
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