Investor Presentaiton

IJMS II COIMAMA INTERNATIONAL JOURNAL of MEDICAL STUDENTS Abstracts Abstracts Abstracts of the 2nd International Academic Medical Congress of Maranhão (COIMAMA) ORAL Presentations 01 Cytotoxic Effects of Euterpe oleracea Mart. in Malignant Hu- man Cells Dulcelena Ferreira Silva; Flávia Castello Branco Vidal; Marcos Antonio Custódio Neto da Silva; Rebeca Costa Castelo Branco; José Andrés Morgado Díaz; Maria do Desterro Soares Brandão Nascimento Introduction: Juçara, Euterpe oleracea Mart., an indigenous fruit from Amazon, is rich in phytochemicals with antioxidant, anti-inflamma- tory and anti-cancer activity. Objectives: This study aims to analyze the effects of the hydroalcoholic extract of the bark, seed and to- tal fruit of juçara in different human malignant cell lines. Methods: Fruits were collected at the Maracana Ecological Park, in São Luís, followed by excicata manufacturing that remains registered in the Herbarium Rosa Mochel from the Nucleus of Biological Studies at the State University of Maranhão. The hydroalcoholic extracts of bark, seed and fruit were all obtained in the Laboratory of Pharmacolo- gy and Psychobiology UERJ. The cell lines used in the tests were MCF-7 and MDA-MB-468 (breast adenocarcinoma) and CACO-2 and HT-20 (colorectal adenocarcinoma). Results: Strains were treated with 10, 20 and 40µg/mL of extracts for 24 and 48 hours. Control MCF-7 cells showed prominent nucleus with evident nucleoli. After treatment with the hydroalcoholic extract from the bark of juçara, the cells showed rounded morphology with retraction of the cyto- plasm. The MTT viability assay showed a reduction in cell viability. After 48 hours, treatment of cells with 20µg/mL of bark extract re- duced cell viability and the cytotoxic effect of treatment with 40μg/ mL extract of the bark was potentiated. Cells treated with 10μg/mL of the bark extract were rounded with consequent reduction in cell volume. The concentration of 20µg/mL of bark extract caused seve- re reduction in volume of the cells and caused the appearance of intracellular vacuoles. The same was observed after treatment with 40μg/mL. Treatment with 40μg/mL of the hydroalcoholic extract of total fruit dramatically changed the morphology of the MCF-7 cells causing vacuolization and lysis with apparent loss of cytoplasmic contents. MTT assay showed a reduction in viability of MCF-7 cells treated with 20 and 40µg/mL after 24 hours of treatment. Analysis by electron microscopy showed the appearance of autophagic vacuoles. Autophagosome protein LC3BII was identified by Western Blotting technique. Conclusion: It is confirmed that the isolated fractions of the bark extract from juçara promotes cellular changes indicative of autophagy from 10μg/mL in 24 hours. The nuclei remained intact, showing no apoptotic features. The data are conclusive for the occu- rrence of autophagy in carcinoma cell line MCF-7 breast treated with hydroalcoholic bark, seed and fruit from juçara. 02 Correlation between Hepatitis Type A and Helicobacter pylori Infection In São Luís Maranhão - Brazil Fernando Félix Calvet Campêlo; José de Macêdo Bezerra; Ro- drigo Duart Martins Souza; Marcos Antonio Custódio Neto da Silva; Graça Maria de Castro Viana; Maria do Desterro Soares Brandão Nascimento Introduction: The infection by Helicobacter pylori occurs in more than half the world's population. Objectives: The objective of this study was to associate infection of Hepatitis type A with Helicobacter pylori in adults of São Luis - MA - Brazil. Methods: The sample consists of 143 patients aged between 17 and 70 years attending the University Hospital Presidente Dutra, Universidade Federal do Maranhão, with complaints of the upper digestive tract, conveyed to Endoscopy Cen- ter and collected medical history simplified, fragments for biopsy of the antral region and venous blood. Results: The positive results for H. pylori infection was 75,5% (108/143), and seroprevalence for Hepatitis A virus was 94,9% (130/137), there was no significant di- fference when compared to sex. The analysis of the association of infection by H. pylori by serology for HAV showed that, of the 134 individuals who have made tests for both infections, 97 (72.4%) were positive for both tests, 3 (2.2%) negative for H. pylori and HAV, 30 (22.4%) only positive for HAV and 4 (3%) only positive for H. pylori. Conclusion: Data presented in this study corroborate the findings in the literature that there is no association of infection by the hepatitis A virus and H. pylori infection. 03 Mapping of Technologies on Leishmania Vaccines through Patent Documents Abner De Oliveira Freire; Emerson Lucas Frazão Sousa; Vitor Fernandes Rios; Lilalea Goncalves Franca; Maria Do Socorro De Sousa Cartágenes; João Batista Santos Garcia Introduction: The genus Leishmania involves intracellular parasi- tes protozoans of monocytic phagocytic system cells, causers of the Leishmaniasis, which may affect skin, mucous membranes or viscera, also being significant between emerging parasitic diseases in the world, affecting widely mammals and presenting ineffective treatment methods. However, vaccines development have been seen as a possible tool to combat the disease. Objectives: Map the exis- ting leishmaniasis vaccines technologies through patent documents. Methods: Searches for patent documents were conducted through INPIⓇ, EspacenetⓇ and The LensⓇ databases between June 3rd and 19th 2015 with "Leishmania", "patent" and "vaccine" as keywords, and data were treated at Microsoft Excel version 2013, analyzing the following criteria: publication year, document depositor institu- tion and its nationality, and the employed technologies according to International Patent Classification. Results: 144 patent documents were recovered, being the deposit increase remarkable over past 10 years, with the highest patents number in 2010. The United States of America were highlighted with 50,3% of depositors institutions and the remaining belong to European Union (14,1%), Brasil (13,4%) and other countries (21,9%). The most frequent technology involves class A61K39 (Medicinal preparations containing antigens or antibo- dies), with 123 documents. Conclusion: Leishmaniasis vaccine deve- lopment embraces global effort including significant participation of Brazil, a country which comprises huge scientific and technological potencial and, in the same time, carries the disease as a severe public health problem. Furthermore, the analysis of new technolo- gies in Leishmania vaccines through patent documents proved to be efficient, because of large amount of information cointained in, such as new technological trends, potentials, progression and industry characteristics, showing new and promising directions for research in the area. 04 Anal exposure ventricular-peritoneal catheter: Case report Taynara Luna de Oliveira; Caroline Carvalho de Araújo; Igor Leonardo Vieira Caetano; Larissa Gomes Farias; Jonas Bran- dão Pereira; Elis Raquel da Silva Araújo Introduction: Ventricular shunt (PVD) is a surgical procedure that establishes a communication between the ventricles and the perito- neum (the membrane covering the abdominal wall) using a catheter. This implies the deviation of cerebrospinal fluid (CSF) into the ab- dominal cavity. PVD is a surgical procedure used primarily to treat a condition called hydrocephalus, which occurs when excess cere- brospinal fluid (CSF) is accumulated in the ventricles of the brain. The most frequent complications of shunt dysfunction and infections are. The most common sites of gastrointestinal perforation are sig- moid, followed by transverse colon and stomach. The Case: Female child, four months old was born with myelomeningocele and hydro- cephalus still detected prenatally. Surgery to dysraphism correction on the third day of life and ventricular-peritoneal shunt (VPS) to the right to 15 days of life was submitted. The mother arrived at the hospital complaining that he had realized the output of clear liquid through the anus child the night before, with rear projec- tion transparent tube through which liquid dripping this after a few hours. He said, even if the baby was more sleepy. On examination, the child was afebrile, alert, depressed anterior fontanelle, without signs of meningeal irritation. The proctoscopy showed the presence of exposed shunt catheter approximately 10 cm from the anal verge. The path-ray showed a perforation through the catheter in the sig- moid segment of the intestine and its path to the anus. The CT scan showed the location of the proximal catheter within the pellucid septum absence of hydrocephalus and signs of depression anterior fontanelle. The child was hospitalized for 15 days when he received antibiotics and was discharged in excellent neurological status, nor- motensive fontanelle and outpatient guidance. Conclusion: Given the above, indicating the surgical removal of the shunt brought a great benefit to the patient by the urgency of the frame. After the with- drawal, the patient had no need of replacement ventricular shunt. 05 Epidemiological Profile of Children 0-4 Years Aged Reported with Meningitis in Araguaína-To, between January 2010 and June 2015. Tamires Lopes Oliveira; Fabiana Silva dos Santos; Ana Bárbara Feliciano Souza Santos. Introduction: Meningitis is a relevant public health problem, res- ponsible for high mortality and morbidity in children, despite the improvement of diagnostic methods and treatments. About 5% to 40% of children die from the disease, and 5% to 30% of the survivors suffer from neurological sequelae. Meningitis is generally caused by infection of viruses, bacteria, fungi, parasites, and protazoa. In the majority of cases, the caused by bacteria. Clinical manifestations vary with age and duration of the disease. Nonspecific symptoms may be present, like myalgia, hypotension, tachycardia, arthralgia, petechiae, fever, irritability and bulging fontanelle. The key to the diagnosis is the cerebrospinal examination, which is analyzed by cell count, chemistry, Gram staining and cultures. Immediate empirical antibiotic therapy is imperative, and must consider the most fre- quent pathogens. Prior to treatment, lumbar puncture and a blood culture should be obtained. Objectives: This study aims to evaluate the epidemiology of children aged 0-4 years, who had been noti- fied with meningitis in Araguaína-TO, between January 2010 and June 2015, confronting the literature. Methods: It is a study epidemiolo- gical, quantitative and retrospective. The electronic health databa- ses SINAN (Sistema de Informação de Agravos de Notificação) were used to collect information about age, gender, lethality and etiology. Analyses were performed using Epilnfo 7 and Excel. Results: During the selected period, 74 cases of meningitis was reported: 37(50%) were children younger than 1 year of age, 37 (50%) male and 37 (50%) female. Four (5,4%) deaths were reported due to complica- tions of meningitis. Regarding the etiology, 54 (72,97%) were viral, 19 (25,68%) bacterial and 1 (1,35%) not specified. The year 2014 stand out as having the largest number of incident cases, with 24 (32,4%) new reported cases. Conclusion: Despite the high number of cases of meningitis in this age group, lethality rate for this cause, proved to be low, at the lower limit found in the literature. The incidence of the disease in children younger than 1 year accounted for half of the cases, possibly reflecting their immaturity of the immune system. There was no difference in the incidence of meningitis among the sexes, as in literature, the male was identified as being the most affected. The literature suggests bacterial etiology as the more fre- quent, however, in this study, the viral was the most incident. 06 The Ethical Perception of Medical Students from a Private College and a Public University in Teresina-PI Evellyn Batista da Silva Flizikowski; Joycce Huanna de Souza Silva; Ravena Vilarinho Santos Soares; Jessica da Silva Prates; Thalyta Batista de Sousa; Edison de Araújo Vale Introduction: Ethical issues has been constant targets of discussions in view of the growing number of occurrences due to the neglect of life, especially in the medical field. This fact constitutes a social concern that could impact on public health in Brazil. Objectives: The aim of this study was to analyze the ethical perception of medical students from a private college and a public university in Teresina- Pl. Methods: The study is descriptive and analytical character, with quantitative approach, performed from a standardized questionnaire after the approval of the 920.192 number of ethics committee and then analyzed using SPSS version 20.0. The students were questio- ned in the period from August to December 2014, as the knowledge of the medical code of ethics, perceptions about the poor ethical behaviour of teachears, change in desing and ethical behaviour com- pared to the beginning of the course, the appropriate time to teach discipline and update frequency as medical ethics. Results: It was analyzed 162 students, from 1, 2, 7 e 8 periods, which 53,09% of private institution and 49,61% of a public institution. Of this total, 92 students were males and 70 were females. As the knowledge of CEM, 124 said they had read in part, 29 never read and 9 read completely. 66,05% had teachers with poor ethical behaviour and 45,06% said physicians teachers struck the CEM. About 143 of the students sur- veyed said they had changed their ethical behaviour when compared to the beginning of the course, 42 prever to have the subject taught in the first half, 51 in all periods, 18 as optional and the remaining 51 students, in other periods. 109 rarely are update, not 18, 17 annually, semi-annually 13 and 5 monthly. Conclusion: Despite the low interest in the knowledge of CEM and medical update, most scholars consi- der it necessary to be given the subject in every semester, report changes in moral behaviour from the beginning of the semester and highlight the bad example of teachers regarding compliance with the CEM and behaviour to ethical and profissional. 07 Epidemiological profile of patients with diabetes mellitus by the Family Health Strategy in the Health Center Djalma Marques, Turu, São Luís, Brazil. Bárbara Lima Ribeiro; Acácia Pinto Morais; Lianna Paula Gu- terres Corrêa; lolanda Margarete De Araújo Rego Introduction: Diabetes mellitus (DM) is not a single disease but a heterogeneous group of metabolic disorders has in common hyper- glycemia, which is the result of defects in insulin action, in insulin secretion or in both. The number of diabetic patients is increasing due to the growing and aging population, greater urbanization, the increasing prevalence of obesity and sedentary lifestyle, as well as the longer survival of patients with diabetes and it is estimated that by 2030 will be 300 million adults with DM. Objectives: Analyze and demonstrate the epidemiological profile of patients with DM followed by the Family Health Strategy (FHS) at the Health Centre Djalma Marques, Turu, São Luís, Brazil in relation to gender, age and current medications, and were taken into account only those provided by the Unified Health System (SUS). Methods: It is a health profile, descriptive and quantitative approach. The survey was con- ducted through the Records Book of Hypertensive the Basic Health Unit (BHU) in question by means of master data Installation and Ope- rating Manual - HIPERDIA in period April 2013 to July 2015. Results: It was observed that 100 of the patients analyzed, 54% were between 61 and 80 years; 34% between 41-60 years; 8% between 81 and 100 years; 2% under 40 years and 2% over 100 years. As to gender, 65% were female. As for medication, 75% make use of Glibenclamide; Metformin 68% and 11% of Insulin. Conclusion: The epidemiological profile of the stroke study shows most female patients. The predomi- nant age group is between 61-80 years. The medication predominant in use is Glibenclamide. This research demonstrates the importance of the ESF program and the free distribution of anti-diabetic drugs to patients as a way to get better adherence to treatment and improved quality of life. It is noteworthy, too, the need for constant improve- ment to preventive actions, thereby reducing hospitalizations, and therefore preventable public expenditure, enabling the use of these resources in other health areas. 08 Epidemiological profile of patients with systemic hyper- tension accompanied by the Family Health Strategy in the S16 INTERNATIONAL JOURNAL of MEDICAL STUDENTS www.ijms.info • 2015 Vol 3 Suppl 1 www.ijms.info • 2015 Vol 3 Suppl 1 II COIMAMA INTERNATIONAL JOURNAL of MEDICAL STUDENTS S17