BioNTech Investor Day Presentation Deck
Delivery formulations
A diversified and rationally designed delivery platform for mRNA medicine
Lipoplex (LPX): mRNA
embedded between lipid bilayers
to form a sandwich like complex
Target:
Lymphoid-resident dendritic cells in
lymphoid compartments body-wide
(spleen, lymph nodes, bone marrow)
Therapeutic applications:
●
Therapeutic cancer vaccines:
Fix Vac, iNeST
1 Grabbe S, et al. Nanomedicine 2016; 11:2723-2734; 2 Kranz LM, et al. Nature 2016; 534:396-401.
Schematic depiction of lipid bilayers¹ Schematic depiction of RNA-lipoplex screening process ¹
DOT MA/DOPE
RNA condensing
Lungs Liver Spleen
RNA (LP)
Fluid
Rigid
formation protocols
Biological testing
+
Variation of cationic lipid-to-nucleotide ratio
Multi-platform engine
Liposome screening
Bioluminescence imaging of BALB/c mice (n=3) after IV injection of Luc-LPX at various charge ratios²
(+):(-) 5:1
(+):(-) 3:1
(+):(-) 1.7:2
(+):(-) 1.3:2
(+):(-) 1:5
Size
D
1
Charge
Ligands
PEG
2.7 x 106
2.0 x 105
2.0 x 105
X
ī
1.2 × 10²
Spleen
Liver
Lungs
BIONTECH
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