MaxCyte IPO Presentation Deck
What is Electroporation?
• Electrical pulses are applied to cells suspended
between two electrodes, which creates an electric
field and polarization of the membrane
At a certain electrical field threshold, openings form in
the cellular membrane (can be simplistically thought
of as pores)
• The size and distribution of these openings depends
on the intensity of the applied electric field and
duration of cell exposure
. The openings allow the entry of macromolecules such
as RNA, DNA or proteins (such as Cas9/guide RNA
complexes) through the cell membrane
July 2021
A key discovery in EP was that the e effects of
electroporation on the cellular membrane are
reversible
. Once the electric field is removed, the membrane has
the capacity to reseal, trapping the molecules that
passed across the membrane within the cytoplasm.
1
3
Cathode (-)
RNA
CONFIDENTIAL
Electrode
DNA
XX
Anode (+)
Protein
2
4
Cell Membrane
Willk
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