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PFS probability (%) Polygenic Scores for Hypothyroidism, Psoriasis Predicted Clinical Efficacy to Immune Checkpoint Blockade IMpassion131 Phase 3 (Breast Cancer) Placebo-paclitaxel (n=220) Atezolizumab-paclitaxel (n=431) 100 90 80- 70- 60 50 40 30 20 10- 0 Negative Phase 3 Studies 5.6 5.7 (95% CI 5.4-6.5) (95% CI 5.4-7.2) 6 9 Overall survival (%) Number at risk Atezolizumab Chemotherapy 100 IMvigor211 Phase 3 (Bladder Cancer) 80- 60- 40- 20- Stratified HR-0.86 (95% CI 0.70-1.05) Not formally tested 12 15 Time (months) Miles, et al. Ann Oncol 32:994, 2021. Overall survival 18 21 Events/ number of patients -Atezolizumab 72/116 Chemotherapy 88/118 24 12 month overall survival rate (95% CI) Median overall survival (months; 95% CI) 11-1 (8-6-15-5) 46-4% (37-3-55-6) 10-6 (8-4-12-2) 41-2% (32-2-50-3) Stratified HR 0-87, 95% CI 0-63-1-21: p-0-41 Leave 0 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 112 100 88 85 82 77 73 58 55 47 118 109 100 95 91 85 82 75 71 65 61 51 47 41 32 28 24 18 15 11 92821 27 Powles, et al. Lancet 391:748, 2018. Genetic variation associated with thyroid autoimmunity shapes the systemic immune response to PD-1 checkpoint blockade Zia Khan ¹, Christian Hammer¹, Jonathan Carroll, Flavia Di Nucci¹, Sergio Ley Acosta¹, Vidya Maiya', Tushar Bhangale¹, Julie Hunkapiller¹, Ira Mellman¹, Matthew L. Albert 1,3, Mark I. McCarthy ¹ & G. Scott Chandler 2 NATURE COMMUNICATIONS | (2021) 12:3355 | https://doi.org/10.1038/s41467-021-23661-4 | www.nature.com/naturecommunications Polygenic risk for skin autoimmunity impacts immune checkpoint blockade in bladder cancer Zia Khan,¹, Flavia Di Nucci", Antonia Kwan", Christian Hammer", Sanjeev Mariathasan", Vincent Rouilly", Jonathan Carroll, Magnus Fontes, Sergio Ley Acosta, Ellie Guardino, Haiyin Chen-Harris, Tushar Bhangale", Ira Mellman ³¹, Jonathan Rosenberg, Thomas Powles, Julie Hunkapiller", G. Scott Chandler", and Matthew L. Albert1.2 PNAS June 2, 2020 117 (22) 12288-12294; first published May 19, 2020; https://doi.org/10.1073/pnas.1922867117 Survival probability 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0.0 Strata PRS(hypoT)-below median+ PRS(hypoT)-above median 0 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 Time (days) impas130/ANabP Hazard Ratios for Atezolizumab Overall Survival Psoriasis PRSS Vitiligo PRSs Atopic Dermatitis PRS Tumor T-cell Effector • ● 0.6 0.7 0.8 0.9 1 1.25 1.5 1.75 HR per unit normalized PRS 95% CI; HR <1 high PRS better Copyright © 2022 23andMe, Inc. 23andMe® 52
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