Cytek Investor Day Presentation Deck
DC1555 KRC Arno
versus spectral
analysis
Aurora analyzer
Option 3B
SSC-H
3.0M-
2.0M-
1.0M-
0
0
1.0M
CD3 PECY5
CD4 QD655
Mloid cells
9.72
wwwphecytes
47.2
HLA-DR PECY7
CD14 QD800
2.0M
FSC-H
3.0M
CD56 BV421
CD45 eFluor 450
CCR7 BV605
CD19 StarBright Violet 610
4.0M
Comp-BV421-A :: CD56
SSC-H
CD2 BV711
CD8 QD705
CD45RA AF488
CD20-biotin streptavidin-QD525
Comp-B/605-A : CD20
10°
105
104
4.0M-
3.0M
2.0M-
1.0M-
0
10°
-10
monocytes
-104
-104
-104
104
Comp-Odot 800-A CD14
0
0
CD19+ CD20+
51.7
10
Comp-eFluor 450-A: CD45
0
CD56+
65.0
CD45+
99.3
10
10
B cells
Comp-StarBright Vio 610-A
105 108
Comp-EV711-A :: CD2
Comp-BV711-A :: CD2
Comp-Qdot 705-A :: CD8
10
10⁰
105
-1042
105
103
-104
NK cells
CD56+
86.4
-104
CD3- CD2+
7.74
-10"
-104
B
0
Cono-BV421-A: CD56
0
™™|™|||||
0
104
CD3+ CD2+
75.1
DN
14.5
104
Comp-PE-Cv5-A: CD3
CD4 T cell
CD8+
32.8
105
104
105
Comp-Qdot 655-A :: CD4
10° 10
A
T
108 107
Fluorochromes
intentionally chosen for
close spectral proximity.
10⁰
Comp-Alexa Fluor 488-A :: CD45RA
Comp-Qdot 525-A :
Comp-Qdot 525-A :: CCR7
105
104
10°
-104
12-color panel comparison
between spectral and
fluorescence lifetime
cytometry.
CD4 T naïve cells
-104
-104
-104
CD4+CD45RA+
28.4
104
Comp-Odot 655-A
0
0
CD4+ CCR7+
44.1
104
Comp-Odot 655-A CD4
CD8+ CCR7+
36.3
CD4
104
105
10°
Comp-Odot 705-A :: CD8
10
10
||||||||||||||
105
108
Comp-Alexa Fuor 488-A :: CD45RA
Comp-PE-Cy7-A :: HLA-DR
Comp-PE-Cy7-A: HLA-DR
108
-104
10
10⁰
104
-104
10
105
-104.
CD8+ CD45RA+
17.0
CD8 T naïve cells
10
-104
-10"
0
Comp-Odot 705-A :: CD8
10
CD4+ HLA-DR+
3.34
CD8+ HLA-DR+
5.91
זיזו
0
Comp-Qdot 655-A CD4
104
105
104
10°
105
Comp-Qdot 705-A: CD8
10
108
10⁰View entire presentation