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Pharma Update

Zilebesiran with best-in-disease potential in hypertension New MoA with tight upstream blockade of AGT pathway and strong early results Zilebesiran (siRNA targeting AGT) Alnylam Roche PHARMACEUTICALS AGT Zilebesiran No AGT synthesis 0 Renin Angl Angli 나이 ABPM ACE GO ABPM Aldosterone 09 ०० Serum AGT change from baseline % -40- -60- -80- -100- 0 Ph I results in hypertension¹ Change in serum angiotensinogen Mean change in 24-h ambulatory diastolic and systolic blood pressure Placebo 10 mg A25 mg 450 mg +100 mg *200 mg 400 mg ▶800 mg Week 8 Week 24 + Change from Baseline (mm Hg) 30- 30- 25- 15- 15- 10- 10- 5- 0.5 1.1 5- 0- 0- -5- -5- -10- -10- -5.6 -15- -7.9 -15- -5.4 -20- -10.9 -10.4 -9.0 -20- -5.7 -25- -25- -12.5 -9.3 -10.8 -30- -30- -35- -40- -16.8 -35- -22.5 -40 Placebo (N=27) Zilebesiran, 200 mg (N=7) Zilebesiran, 400 mg. (N=8) Zilebesiran, 800 mg (N=8) Zilebesiran, 200 mg (N=6) Zilebesiran, 400 mg (N=8) Zilebesiran, 800 mg (N=8) 12 24 Study week Diastolic blood pressure Systolic blood pressure • siRNA targeting angiotensinogen, the precursor of all angiotensin peptides may avoid RAAS escape • Consistent + durable blood pressure control with potential for improved adherence • Positive Ph I results: >90% reduction of serum AGT for up to 6 months at single SC dose of zilebesiran ≥100mg Decreases in systolic blood pressure (>10 mm Hg) and diastolic blood pressure (>5 mm Hg) by week 8 and sustained at 24 weeks at single SC dose of zilebesian ≥200mg • Well tolerated, only mild-to-moderate injection site reactions and no TRAES, hypotension or significant alterations of renal/liver function 1 Desai et al. N Engl J Med 2023;389:228-38; MoA-mode of action; siRNA-small interfering RNA; SC=subcutaneous; RAAS-renin angiotensin aldosterone system; AGT-angiotensinogen; Angl/II-angiotensin I/II; ACE-angiotensin-converting enzyme; ABPM-ambulatory blood pressure monitoring; SoC-standard of care; TRAES-treatment related serious adverse events; zilebesiran in partnership with Alnylam Pharmaceuticals 116
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