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Investor Presentaiton

II. Carragelose® significantly inhibit SARS-CoV-2 replication in tissue culture New Data published on August 21st from independent group in Argentina/USA SARS-CoV-2 virus replication inhibition (Log10 TD50) 7.00 marino med 6.00 5.00 4.00 3.00 2.00 1.00 0.00 Untreated 600 ug/ml 60 ug/ml 6 ug/ml 0.6 ug/ml Placebo (P2) SARS-COV-2 viral titer after treatment with iota carrageenan and P2 (3 replicates per treatment) Composition 1.2 mg/mL iota-carrageenan, 5 mg/mL sodium chloride, pH 6-7. Vero E6 were pre-treated with dilutions of Iota-Carrageenan with sample P2 (placebo without iota-carrageenan) to get 600 μg/mL, 60 μg/mL, 6 μg/mL, and 0.6 μg/mL final iota- carrageenan concentration for 2 h. After a 2 h pre- treatment, cells were infected with SARS-CoV-2 and incubated for 48h in the presence of the same dilutions of iota-carrageenan. Supernatants were harvested and virus yield determined by an end point dilution assay (TCID50). Controls consisted of untreated infected cells or infected cells treated with P2 (no iota-carrageenan). Results were determined using the Reed and Muench formula and expressed as log TCID 50/mL. Dotted line shows the limit of detection (LOD). Testing of samples was performed in triplicate. CarrageloseⓇ reduces SARS-CoV-2 virus replication by more than 99,99% or 4,75logs even when the product is diluted by a factor of 1:200 Source: https://www.biorxiv.org/content/10.1101/2020.08.19.225854v1 6
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