Akouos IPO Presentation Deck

The High Transduction Efficiency of AAVAnc80 Coupled with Local Delivery to the Inner Ear Compartment Allows for a Dual Vector Approach for Larger Transgenes ITR 25 ITA الے promoter 5 GFP cDNA promoter 5' GFP cDNA SD ITR ITR SA Transduction ITR t ITR SA DNA sequence alignment Recombination Transcription Splicing polyA TR 3º GFP cDNA H 3* GFP cDNA GFP mRNA Translation polyA ITR Percentage of Inner Hair Cells Expressing Transgene Threel Weeks Following Administration of Dual AAVAnc80 Vectors Inner Hair Cell Transduction (%) 100 80- 60 40- 20- 0- 7.2E11 vg 0.06 Monkey 1 Monkey 2 Monkey 3 Monkey 4 0.5 4 32 Cochlear Frequency Position (kHz) AKOUOS Functional protein Ability to deliver transgenes that are larger than 5 kb in size creates the potential for broader treatment of genetically-driven hearing loss CONFIDENTIAL

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