Cytek Investor Day Presentation Deck

A Selection of Application Areas for FSP CYTEK TRANSCEND THE CONVENTIONAL Immuno-oncology blood 803 EMERGING DIAGNOSTIC TOOLS AND TECHNIQUES | NOVEMBER 5, 2020 One Tube 24 Color Full Spectral Flow Cytometry and Multi-Dimensional Software to Study the Maturation Pattern and Antigen Expression of the Myeloid Man Chen Wang MD Mining Fun Wang Mewel Gong Juny Zhen Xuying W Kun Zhan Pehua Lu MO Pathology & Laboratory Medicine Division Haber Yanda Ludaper hospital Langlang, China Pathology & Labiry Medicine Din Habe Yands Lu Dapel Heng Chi "Pathology & Laboratory Medicine Dion Hebel Yande Ludope Hospital, Langtang China Yand LuDepe H Langling China 13:13 httpdol.org/10.1182/blood-2020-140000 Introduction Flow cytometry(FC) plays an important role in the diagnosis of hematologic diseases and the study of cell maturation Spectral multicolor flow cytometry(MFC) has shown an advantage over traditional FC that more fuorescent markers could be detected simultaneously, more antigen combinations could be made and the expression of cells could be scrutinized. However, published studies focused on lymphocyte subsets and the differentiation between hematogones and B-acute lymphoblastic leukaemia/ymphoma(ALL) meal residual disease (MRO) detection, and the there are few studies on myeloid development and expression. Besides, more powerful and cutting-edge software are needed for complicated combinations from SMFC because traditional dot plots are unable to meet the demand of analysis. Here we design a one-tube 24-color panel combining with the multidimensional data analysis software to study the expression and maturation of normal and malignant myeloid cells including minus subgroups. We hope to improve the sensitivity of MRD by FC and explore more information about myeloid diseases, finally promote the development of anficial intelligence(Al) in clinical FC diagnosis Methods: the one-tube 24-color panel was designed according to our experience and Euroflow recommendation, it is composed of backbones including CD45 and myeloblast markers CD34, CD117 and HLA-DR, adding myeloid markers CD33. CD13, CO371. CD1S, CD64 CD11c/CD14, CD36 and CD11b, routine leukaemia associated immunophenotyping(AIP) or different from normal(DFN) markers CYTEK TRANSCEND THE CONVENTIONAL Immuno-deficiencies PLOS ONE OND RESEARCH ARTICLE Natural killer cell phenotype is altered in HIV-exposed seronegative women Nancy Q Zhao Elane Vendrame Anne-Maud Fair, Christof Saker Thanay Rangana Mary Annie Claude Labbe, Femend Goedo, Johanne Poudrier Susan Holmes, Michel Roger Cas Published September 1, 2020 ps dol org/10.1371/joumal pone 0238347 Alastad Materias and methods Rendh Discussion Supporting inalin Adm Palaces Reader Comments Figures Authors Metrics Comments Media Coverage Abstract Highly expregative HESN) individuals presenta unique setting to study mechanisms of protection against HIV acquistan As natural kler (K) cell activation and function have been implicated as a corelate of protection in HESN individuals, we ought to better understand the features of NK calls that may confer protection. We used mass cytometry phenotypically proe NK cells from a cohort of Berinese sex workers and healthy controls. We found at Nata from HESN saman had increased asprassion of NA NK30 ULRB1, as wet as the Fc receptor CO16, and decreased expression of DNAM, CO ssments of NK cels om haathy donors again and Nodd Usino hectatel te Signed 14 teas autologous HIV infected CD4" Tools, wo and Siglec- cells had improved functional activity Further we found that NK calls from HESIN wameended towards increased antibody-dependent cellular cytotoxicity (ADCC) activity activity correlated with increased CD18 expression Overall, we idently features of NK oss in HESN women that may contribute to protection from HIV infection Follow up studies with largar cohorts are wenanted to conkm these findings Figures BERGARAGE 555555555 ... Flow Cytometry Cell Analysis Allergy & Immunology Androgen receptor signaling promotes Treg suppressive function during allergic airway inflammation Vive D. Gandhi, Jacqueline-Yvonne Cephus,' Allison E. Norlander,' Nowrin U. Chowdhury,² Jian Zhang,¹ Zachary J. Ceneviva, Elie Tannous,¹ Vasiliy V. Polosukhin, Nathan D. Putz,' Nancy Wickersham,' Amrit Singh, Lorraine B. Ware,' Julie A. Bastarache,¹ Ciara Peebles ,12 and Dawn C. Newcomb¹2 Shaver, Hong Wei Chu, R. Stokes Authorship note: VDG and JYC contributed equally to this work. Published January 13, 2002- More info View PDF Women have higher prevalence of asthma compared with men. In asthma, allergic airway inflammation is initiated by IL-33 signaling through ST2, leading to increased IL-4, IL-5, and IL- 13 production and eosinophil infiltration. Foxp3 Tregs suppress and ST2- Trega promote allergic airway inflammation, Clinical studies showed that the androgen dehydroepiandrosterone (DHEA) reduced asthma symptoms in patients, and mouse studies showed that androgen receptor (AR) signaling decreased allergic airway inflammation. Yet the impact of AR signaling on lung Tregs remains unclear. Using AR-deficient and Foxp3 fate- mapping mice, we determined that AR signaling increased Treg suppression during Alternaria extract (Alt Ext; allergen) challenge by stabilizing Foxp3+ Tregs and limiting the number of ST2- ex-Trege and IL-13- Th2 cells and ex-Trega. AR signaling also decreased Alt Ext-induced ST2+ Tregs in mice by limiting expression of Gata2, a transcription factor for ST2, and by decreasing Alt Ext-induced IL-33 production from murine airway epithelial cells. We confirmed our findings in human cells where 50-dihydrotestosterone (DHT), an androgen, decreased IL-33-induced ST2 expression in lung Tregs and decreased Alt Ext-induced IL-33 secretion in human bronchial epithelial cells. Our findings showed that AR signaling stabilized Treg suppressive function, providing a mechanism for the sex difference in asthma. Infectious Disease RESEARCH ARTICLE Successful treatment of COVID-19 with remdesivir in the absence of humoral immunity > Matthew S Buckland, James Galloway, Caoimhe Nic Fhogartaig, Luke Meredith, Nicholas M. Provine, Stuart Bloor, Ane Ogbe, Wioleta M. Zelek, Anna Smielewska, Anna Yakovleva, Tiffeney Mann, Laura Bergamaschi, Lorinda Turner, Frederica Mescia, Erik J.M. Toonen, Carl-Philipp Hackstein, Hossain Delowar Akther, Vinicius Adriano Vieira, Lourdes Ceron-Gutierrez, Jimstan Periselneris, Sorena Kiani- Alikhan, Sofia Grigoriadou, Devan Vaghela, Sara E. Lear, Estee Torok, William L Hamilton, Josh Quick, Joanne Stockton, Peter Nelson, Michael Hunter, Tanya I Coulter, Lisa Devlin, John Bradley, Ken Smith, Willem Ouwehand, Lise Estcourt, Hell Harvala Simmonds, Dave Roberts, lan Wilkinson, Nick Screaton, Nick Loman, Paul Lyons, Rainer Doffinger, Paul Morgan, lan Goodfellow, Paul Klenerman, Paul Lehner, Nick Matheson, James Thaventhiran Human Publications Successful treatment of COVID-19 with remdesivir in the absence.... Immunotherapy blood advances IMMUNOBIOLOGY AND IMMUNOTHERAPY | SEPTEMBER 8, 2020 Charge-altering releasable transporters enable phenotypic manipulation of natural killer cells for cancer immunotherapy Aaron J. Wilk, Nancy Lynn-Benner Weidenbacher, Rosemary Vergara, Ole A. W. Haabeth, Ronald Levy, Robert M. Waymouth, Paul A. Wender, Catherine A. Blish Human Blood Adv (2020) 4 (17): 4244-4255. https://doi.org/10.1182/bloodadvances 2020002355 Publications Charge-altering releasable transporters enable phenotypic manipulation of natural killer cells 15