First Busey Results Presentation Deck

4Q23 Earnings Investor Presentation Previously Announced 4Q23 Balance Sheet Repositioning Securities Sold Visa Class B Common Shares Sold Net Gain from combined transactions Use of Proceeds Net Interest Income Impact Regulatory Capital Impact ¹ Non-GAAP, see Appendix BUSE announced a two-part balance sheet repositioning strategy during 4Q23 ▪ $110 million of available-for-sales securities sold Pre-tax loss of $5.3 million Approximate yield of 1.56% ■ I First Busey Corporation | Ticker: BUSE ■ ■ ■ ▪ Immediate positive impact on consolidated stockholders' equity and book value per share ▪ Expected to be accretive to capital and earnings per share in future periods Sold all 16,878 of previously held shares of Visa Class B common stock Pre-tax gain of $5.5 million Proceeds deposited into an interest-bearing account at the Federal Reserve yielding 5.40% Anticipate reinvesting the proceeds into loan and investment security opportunities over time Expected to increase net interest income by approximately $4.3 million on annualized run rate basis Repositioning actions improves net interest margin run rate by approximately 4 bps I Risk-based regulatory capital ratios will increase modestly as a result of the proceeds rotating into lower risk-weighted assets 28 B

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